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ISCT Webinar - A Comparative Analysis of Serum and Serum-Free Cultures (hMSC)

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ISCT Webinar: A Comparative Analysis of Using Serum and Serum-Free Culture Systems for Generating Therapeutic Grade Human Mesenchymal Stem Cells

Sponsored by Irvine Scientific 

Date: Wednesday, October 14, 2015


  • Jessie H.-T. Ni, PhD, Chief Scientific Officer, Irvine Scientific, USA


  • Cyndi Kwan, PhD, Senior Scientist, R&D Department, Irvine Scientific, USA

About the Webinar:

Since the early days of cell culture, serum has been an integral component in cell culture media by providing cells with a rich source of diverse nutrients. While serum may currently be acceptable for clinical human mesenchymal stem/ stromal cell (MSC) culture, securing a reliable source and supply of clinical grade serum is becoming an increasing concern. Furthermore, lot-to-lot variability of serum due to its complex mixture of undefined and variable constituents poses a challenge for translational and human clinical studies utilizing ex-vivo MSC culture. Therefore, a serum-free culture system can improve the generation of high quality cells without depending on serum, thus facilitating the development of more robust cellular therapies. This webinar will review the differences between serum-containing and serum-free MSC culture systems (such as autologous vs. allogeneic and application considerations) and when and how to implement the transition to a serum-free medium.


Learning Objectives:

  • Comparison of the  advantages and disadvantages of serum-containing and serum-free media systems 

  • When and how to implement the transition to serum-free medium

  • Learn key protocol differences between using serum-containing and serum-free media

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